Official websites use. Share sensitive information only on official, secure websites. Conceptualization, D. A comprehensive cellular anatomy of normal human prostate is essential for solving the cellular origins of benign prostatic hyperplasia and prostate cancer.
The tools used to analyze the contribution of individual cell types are not robust. Immunohistochemistry with newly derived cell type-specific markers revealed the distribution of each epithelial and stromal cell type on whole mounts, revising our understanding of zonal anatomy.
Based on discovered cell surface markers, flow cytometry antibody panels were designed to improve the purification of each cell type, with each gate confirmed by scRNA-seq. The molecular classification, anatomical distribution, and purification tools for each cell type in the human prostate create a powerful resource for experimental design in human prostate disease. Using single-cell RNA sequencing, immunofluorescence, and flow cytometry, Henry et al.
They identify two additional epithelial cell types enriched in the prostatic urethra and proximal prostatic ducts. The design of novel therapies against disease relies on a deep understanding of the identity and function of each cell type within an organ. A three-dimensional cellular anatomy of normal organs is necessary to better understand the processes of age-related repair and disease.
These efforts have been largely driven by advances in single-cell sequencing to identify cell type and imaging technologies to identify cell location. Because of the challenges with procurement of fresh normal human organs and the pronounced anatomical differences between mouse and human prostate, considerable gaps remain in our understanding of the functions of specific cell types in prostate disease.
