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Official websites use. Share sensitive information only on official, secure websites. Lead contact; to whom correspondence should be addressed: arguello ciml. Energetic metabolism reprogramming is critical for cancer and immune responses. Current methods to functionally profile the global metabolic capacities and dependencies of cells are performed in bulk.

SCENITH is designed to perform metabolic studies ex vivo , particularly for rare cells in whole blood samples, avoiding metabolic biases introduced by culture media. We analyzed myeloid cells in solid tumors from patients and identified variable metabolic profiles, in ways that are not linked to their lineage nor their activation phenotype.

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SCENITH ability to reveal global metabolic functions and determine complex and linked immune-phenotypes in rare cell subpopulations will contribute to the information needed for evaluating therapeutic responses or patient stratification. Applying SCENITH directly to whole blood and human tumor samples they identify metabolic differences between tumor and juxta-tumor associated immune cells. Energetic metabolism comprises a series of interconnected biochemical pathways capable of using energy rich molecules to produce ATP.

Aerobic glycolysis not only supports proliferation but also cell survival in hypoxic conditions. Immune cells are specially equipped to migrate into peripheral tissues and to adapt to the change in microenvironment. Competition for glucose within the tumor microenvironment influences cancer progression and the anti-tumoral immune response by regulating metabolism and function in both tumoral cells and tumor-infiltrating lymphocytes TILs Chang et al.

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The presence of effector immune cells into the target tissue is a predictive marker of response to cancer immunotherapy Galon et al. The success of immunotherapies is restricted to a relatively small proportion of patients, as it requires a functional and metabolically competent immune system to respond to treament Antonia et al. Consequently, there is a great need for a simple immuno-metabolic profiling method for complex samples that can be utilized to stratify patients before treatment and to monitor responses after immunotherapies.

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