Official websites use. Share sensitive information only on official, secure websites. Correspondence: Dr. E-mail: hdimke health. However, this hypothesis has not been confirmed experimentally. However, no direct evidence for this hypothesis has been presented. In vitro , gentamicin failed to activate the CaSR.
Gentamicin is an aminoglycoside antibiotic used to treat Gram-negative bacterial infections, but its use can be complicated by severe side effects such as nephrotoxicity and ototoxicity. Gentamicin has been reported to activate the CaSR. Surprisingly, gentamicin-induced calciuresis was unaltered in mice with gain-of-function mutations in the CaSR or Cldn14 -deficient mice and gentamicin failed to activate the CaSR in vitro , suggesting that gentamicin does not drive calciuresis through the CaSR-CLDN14 pathway.
All animal experiments were approved by the animal welfare committee of the University of Southern Denmark permits and or the University of Alberta permit AUP Spot urine was collected at baseline and at 2 and 4 hours after injection with bladder massage. Animals were anesthetized with 1. Mice were euthanized by cervical dislocation and kidneys isolated. The average age was 10 weeks for both genotypes. Spot urine was collected at baseline and 2 and 4 hours after injection. After euthanasia through cervical dislocation, kidneys were isolated.
After 4 hours, animals were anesthetized with 1. Mice were then euthanized by cervical dislocation and kidneys isolated. Mice were euthanized by decapitation. Mice were housed in metabolic cages with ad libitum access to water and standard rodent chow. Mice were anesthetized with a lethal ip injection of pentobarbital and subsequently euthanized by cervical dislocation.
Animals were equally assigned to either standard rodent diet containing 0. KG, Lage, Germany or a 2. Subsequently, all mice were placed in metabolic cages and continued receiving the assigned injection daily for another 6 days; hour urine was collected daily after the second injection until the final injection.
